Polysome profiling

Polysome profiling is a biochemical technique used to analyze the translation of mRNA into proteins within cells. This method provides insights into how many ribosomes are engaged in translating a specific mRNA molecule, which can be indicative of its translational activity and overall protein synthesis. Here’s a brief overview of the process and its applications: 1. **Preparation**: Cells are lysed to release their contents, including ribonucleoprotein complexes consisting of mRNA and ribosomes (polysomes).