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Peak calling refers to a bioinformatics process used primarily in the analysis of high-throughput sequencing data, particularly in studies involving ChIP-sequencing (ChIP-seq), RNA-sequencing (RNA-seq), and other types of genomic assays. The main goal of peak calling is to identify regions of the genome where there is a significant enrichment of reads that indicate the presence of biological features, such as protein-DNA interactions, transcription factor binding sites, or open chromatin regions.

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