Nested polymerase chain reaction

Nested polymerase chain reaction (nested PCR) is a modification of the standard polymerase chain reaction (PCR) technique that is designed to increase the specificity and sensitivity of the amplification of a target DNA sequence. It involves two consecutive rounds of PCR using two sets of primers. ### Steps Involved in Nested PCR: 1. **First Round of PCR**: - The first set of primers (outer primers) is used to amplify a larger target region of the DNA.