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Mothur is a software package designed for the analysis of microbial communities, particularly those defined by DNA sequence data from high-throughput sequencing technologies, such as 16S rRNA gene sequences. It was developed to provide a comprehensive and user-friendly tool for researchers studying microbial ecology and diversity. Key features of Mothur include: 1. **Versatility**: It supports various steps in the analysis pipeline, including data preprocessing (e.g.

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