Eadie–Hofstee diagram

The Eadie–Hofstee diagram is a graphical representation used in biochemistry and enzymology to analyze enzyme kinetics, particularly to determine parameters such as the maximum reaction rate (V_max) and the Michaelis constant (K_m) of an enzyme-catalyzed reaction. The Eadie–Hofstee plot is derived from the Michaelis-Menten equation, which describes the rate of enzymatic reactions as a function of substrate concentration.