COLD-PCR

COLD-PCR, or Co-amplification at Lower Denaturation temperature-PCR, is a molecular biology technique that enhances the detection of rare DNA mutations in a sample by increasing the specificity of the amplification process. It allows for the preferential amplification of mutated sequences over wild-type sequences during polymerase chain reaction (PCR). The key features of COLD-PCR include: 1. **Lower Denaturation Temperature**: COLD-PCR is performed at a lower denaturation temperature than conventional PCR.