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BamHI is a type II restriction enzyme that is commonly used in molecular biology for DNA manipulation and cloning. It recognizes a specific DNA sequence and cleaves the DNA at that site. The recognition sequence for BamHI is the palindromic sequence 5'-GGATCC-3'. When BamHI cuts DNA, it produces sticky (or cohesive) ends, which are single-stranded overhangs that can facilitate the ligation of DNA fragments during cloning experiments.

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