Protein crystallization

Protein crystallization is a laboratory technique used to form well-ordered crystals of proteins. This process is essential for studying the three-dimensional structures of proteins using X-ray crystallography, a powerful method for determining atomic arrangements in biological macromolecules. The main steps involved in protein crystallization typically include: 1. **Protein Purification**: Before crystallization, the protein of interest must be isolated and purified. This can involve techniques such as affinity chromatography, ion-exchange chromatography, and gel filtration.