Photoactivated localization microscopy

Photoactivated localization microscopy (PALM) is a super-resolution imaging technique used in fluorescence microscopy to achieve spatial resolution beyond the diffraction limit of light. This technique is particularly important for visualizing cellular structures and protein interactions at the nanometer scale. Here's how it works: 1. **Photoactivation**: In PALM, a population of fluorescent molecules is initially in a dark or non-fluorescent state.